Sexually Transmitted Infections (STI) Testing

Syphilis serology:

• Treponema pallidum Assay – IgM & IgG antibody detection
• RPR screening test, qualitative and quantitative ( special requests only)
• TPPA confirmatory test
• VDRL, qualitative and quantitative (cerebral spinal fluids only)

Chlamydia, Gonorrhea and Trichomonas:

• Nucleic acid amplification testing (NAAT)
• Gonorrhea culture

Human Immunodeficiency Virus (HIV):

• 4th Generation Antigen/Antibody Immunoassay detection
• HIV 1 and 2 differentiation Immunoassay confirmation
• Geenius Confirmation

Hepatitis C Virus (HCV):

• HCV Antibody Immunoassay Detection
• HCV Viral Load – quantitative

Herpes Simplex Virus I & II (HSV) and Varicella Zoster Virus (VZV):

• Multiplex PCR Assay

Molecular Assays by Real Time polymerase Chain Reaction (PCR)

• Bordetella pertussis, parapertussis, or holmseii Multiplex Assay
• Carbapenemase Resistant Organism Testing – multiple assays for resistance mechanisms
• Chikungunya, Dengue, Zika Virus Multiplex Assay (requires consultation with Office of Infectious Disease Epidemiology – OIDE)
• Coxiella burnetii (requires consultation with OIDE)
• Eastern Equine Encephalitis (EEE) and West Nile Virus (WNV) (animal organs and/or mosquitoes)
• Ebola virus (requires consultation with OIDE)
• Foodborne outbreaks for: Salmonella, Shigella, Shigatoxin-producing E. coli, Listeria
• Influenza A and B typing, Influenza A subtyping (H1N1 pandemic, H3N2); Influenza B genotyping (B/Yamagata, B/Victoria). Detection of avian strains (H5N1 and H7N9) (requires consultation with OIDE)
• CDC Respiratory Virus Panel with respiratory targets (Panel 1-Respiratory Syncytial Virus (RSV), Human Metapneumovirus, Rhinovirus, Parainfluenza 1 &3, and Adenovirus. Panel 2- Parainflunenza virus 2 & 4, Human coronaviruses 229E, Oc43, HKU1, NL63)
• Herpes Simplex 1 and 2 viruses, Varicella Zoster Virus Multiplex Assays
• Measles and Mumps viruses
• Mycobacterium tuberculosis and Mycobacterium avium complex Multiplex Assay
• Norovirus type GI and GII Multiplex Assay
• Novel Coronavirus (requires consultation with OIDE)
• Potential agents of bioterrorism (See our Laboratory Preparedness page)
•  2019 SARS-CoV-2 Assay
• 2019 SARS-CoV-2, Influenza A, B Multiplex Assay
• Monkeypox PCR Assay

Bacteriology (identification and susceptibility)

• Stool Culture
• Urine Culture
• Strep Culture
• Wound or Miscellaneous Culture
• Carbapenemase Detection – modified Carbapenemase Inactivation Method (mCIM)(Rachel -Deleted CIDT)
• Confirmation of Enteric Pathogens – Salmonella species. Shigella species, Shiga-toxin-producing E. coli (STEC), Listeria monocytogenes, Vibrio species
• Antibiotic Susceptibility Testing (Vitek II Compact)
• Legionella Urine Antigen

Clinical Outbreak Surveillance:

• Campylobacter species
• Listeria monocytogenes
• Salmonella
• Shigella
• Vibrio spp.
• Shiga-toxin producing E. coli (STEC)
• 2019 SARS Coronavirus

Mycobacteriology:

• Specimen digestion and concentration
• AFB smear (Auramine O or Kinyoun) and culture
• Mycobacterium tuberculosis complex (MTB) Antimicrobial susceptibility testing
• PCR for MTB complex and Mycobacterium avium complex (MAC)
• Quantiferon Plus

Food Microbiology (Epidemiologic/Outbreak Investigation only):

• Bioterrorism Agents – culture and Real Time PCR
• Bacillus cereus – culture
• Listeria species – Culture and WGS
• Salmonella species – Culture, PCR and WGS
• Shigatoxin–producing Escherichia coli – Culture, PCR, serotyping and WGS
• Shigella species – Culture, PCR and WGS
• Staphylococcus aureus – Culture
• Vibrio species – Culture Confirmation and WGS
• Campylobacter – Culture Confirmation and WGS

Immunoassays/Antigen/Antibody:

• West Nile Virus (WNV); Saint Louis Encephalitis (SLE); IgM Microsphere immunoassay (human serum and spinal fluid)
• WNV and Eastern Equine Encephalitis (EEE) — IgM Enzyme immunoassay performed on chicken blood, for detection of WNV and EEE in chickens placed around areas of high mosquito prevalence (in summer months). Also known as "sentinel chicken" program.
• Rabies detection by Direct Flourescent Antibody (DFA) from brain stem and cerebellum (requires OIDE consultation) and Rabies PCR for confirmation of indeterminate cases.

Tests Performed by the State Clinic Labs:

• Urine Dip Stick
• Pregnancy – Urine
• HIV Rapid
• Direct Gram Stain for Gonorrhea
• KOH Prep
• Hemocue
• Blood Lead

Visit our Laboratory Preparedness page for testing forms and procedures related to agents of biological and chemical terrorism.

Return to the Delaware Public Health Laboratory home page.

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